杀灭院内耐药致病菌抗菌凝胶
杀灭院内耐药致病菌抗菌凝胶
副标题：英开发可杀灭院内耐药致病菌的抗菌凝胶
作者：伊文 来源：生物谷 日期：2014-08-20
近日，来自英国贝尔法斯特女王大学的研究人员通过研究揭示了他们在抵御医院强耐药性菌株上的一大突破，相关研究刊登于国际杂志Biomacromolecules上。
文章中，研究者开发了一种新型的抗菌凝胶，这种凝胶可以利用天然的蛋白质有效杀灭院内的铜绿假单胞菌、大肠杆菌及金黄色葡萄球菌，遏制其引发的院内感染。这种抗菌凝胶可以破坏细菌的外膜，比如生物被膜等。
研究者Garry Laverty教授表示，当细菌吸附到医疗器械表面上，比如髋关节置换器和导尿管等，细菌就会产生生物被膜（一种粘液状的物质），生物被膜就可以保护细菌细胞免受抗生素的杀灭作用，从而就产生了耐药性的细菌，随着细菌的生长及扩散就会引发严重的、难以治愈的感染性疾病。
这项研究中，研究人员开发的新型凝胶可以靶向杀灭院内的耐药性菌株，这种凝胶中包含有天然的多肽（天然蛋白质），其相同的成分可以形****类的机体组织；研究人员在实验室对这些多肽分子进行修饰后使其形成凝胶物质，从而发挥快速杀灭细菌的作用。
原文摘要：
Ultrashort Cationic Naphthalene-Derived Self-Assembled Peptides as Antimicrobial Nanomaterial
Self-assembling dipeptides conjugated to naphthalene show considerable promise as nanomaterial structures, biomaterials, and drug delivery devices. Biomaterial infections are responsible for high rates of patient mortality and morbidity. The presence of biofilm bacteria, which thrive on implant surfaces, are a huge burden on healthcare budgets, as they are highly resistant to current therapeutic strategies. Ultrashort cationic self-assembled peptides represent a highly innovative and cost-effective strategy to form antibacterial nanomaterials. Lysine conjugated variants display the greatest potency with 2% w/v NapFFKK hydrogels significantly reducing the viable Staphylococcus epidermidis biofilm by 94%. Reducing the size of the R-group methylene chain on cationic moieties resulted in reduction of antibiofilm activity. The primary amine of the protruding R-group tail may not be as readily available to interact with negatively charged bacterial membranes. Cryo-SEM, FTIR, CD spectroscopy, and oscillatory rheology provided evidence of supramolecular hydrogel formation at physiological pH (pH 7.4). Cytotoxicity assays against murine fibroblast (NCTC 929) cell lines confirmed the gels possessed reduced cytotoxicity relative to bacterial cells, with limited hemolysis upon exposure to equine erythrocytes. The results presented in this paper highlight the significant potential of ultrashort cationic naphthalene peptides as future biomaterials.
关键字： 抗菌凝胶,院内感染,耐药性
信源地址：http://infect.cmt.com.cn/detail/572485.html
副标题：英开发可杀灭院内耐药致病菌的抗菌凝胶
作者：伊文 来源：生物谷 日期：2014-08-20
近日，来自英国贝尔法斯特女王大学的研究人员通过研究揭示了他们在抵御医院强耐药性菌株上的一大突破，相关研究刊登于国际杂志Biomacromolecules上。
文章中，研究者开发了一种新型的抗菌凝胶，这种凝胶可以利用天然的蛋白质有效杀灭院内的铜绿假单胞菌、大肠杆菌及金黄色葡萄球菌，遏制其引发的院内感染。这种抗菌凝胶可以破坏细菌的外膜，比如生物被膜等。
研究者Garry Laverty教授表示，当细菌吸附到医疗器械表面上，比如髋关节置换器和导尿管等，细菌就会产生生物被膜（一种粘液状的物质），生物被膜就可以保护细菌细胞免受抗生素的杀灭作用，从而就产生了耐药性的细菌，随着细菌的生长及扩散就会引发严重的、难以治愈的感染性疾病。
这项研究中，研究人员开发的新型凝胶可以靶向杀灭院内的耐药性菌株，这种凝胶中包含有天然的多肽（天然蛋白质），其相同的成分可以形****类的机体组织；研究人员在实验室对这些多肽分子进行修饰后使其形成凝胶物质，从而发挥快速杀灭细菌的作用。
原文摘要：
Ultrashort Cationic Naphthalene-Derived Self-Assembled Peptides as Antimicrobial Nanomaterial
Self-assembling dipeptides conjugated to naphthalene show considerable promise as nanomaterial structures, biomaterials, and drug delivery devices. Biomaterial infections are responsible for high rates of patient mortality and morbidity. The presence of biofilm bacteria, which thrive on implant surfaces, are a huge burden on healthcare budgets, as they are highly resistant to current therapeutic strategies. Ultrashort cationic self-assembled peptides represent a highly innovative and cost-effective strategy to form antibacterial nanomaterials. Lysine conjugated variants display the greatest potency with 2% w/v NapFFKK hydrogels significantly reducing the viable Staphylococcus epidermidis biofilm by 94%. Reducing the size of the R-group methylene chain on cationic moieties resulted in reduction of antibiofilm activity. The primary amine of the protruding R-group tail may not be as readily available to interact with negatively charged bacterial membranes. Cryo-SEM, FTIR, CD spectroscopy, and oscillatory rheology provided evidence of supramolecular hydrogel formation at physiological pH (pH 7.4). Cytotoxicity assays against murine fibroblast (NCTC 929) cell lines confirmed the gels possessed reduced cytotoxicity relative to bacterial cells, with limited hemolysis upon exposure to equine erythrocytes. The results presented in this paper highlight the significant potential of ultrashort cationic naphthalene peptides as future biomaterials.
关键字： 抗菌凝胶,院内感染,耐药性
信源地址：http://infect.cmt.com.cn/detail/572485.html
没有找到相关结果
已邀请:
1 个回复
尘埃1969
赞同来自: